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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Molecular and metabolic pathways mediating curative treatment of a non-Hodgkin B cell lymphoma by Sindbis viral vectors and anti-4-1BB monoclonal antibody

Fig. 4

Sindbis virus plus α4-1BB combination induced Th1 differentiation and IFNγ production. a IFNγ Elispot analysis of splenocytes harvested at day 2, 7, 14 and 28 from all groups as indicated. Upper panel, IFNγ Elispot image on day 7 after treatment. 1,2,3: three individual mice. Lower panel, IFNγ spots number from indicated groups over the course of treatment (2 × 105 splenocytes per well). No stimulator was added. b IFNγ production from CD4/CD8 T cell population in splenocytes and purified CD4/CD8 T cells. All groups were cultured in media for 5 h in the presence of brefeldin A. c IFNγ production from purified CD4 T cells at different stimulation conditions. d Upregulated Th1 pathway gene set under SV, α4-1BB and SV + α4-1BB stimulation. Expression values are shown by Z-score. Genes are hierarchically clustered by one minus Pearson correlation (day 7). e T-bet expression for all groups as indicated. f, EOMES expression for all groups as indicated. CD8 T cell gated. e, f day 7 after treatment. *, p < 0.05; **, p < 0.01, ****, p < 0.0001

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