Fig. 1

Characteristics of the growth and progression of tumors developed after sc implantation of IL-30-silenced PIN-SCs in WT and IL-30KO mice. a Flow cytometric profiling of phenotype markers expressed by PIN-SCs. Blue profiles illustrate the expression of specific markers, while black profiles represent isotype controls. Each panel is representative of three independent experiments. b Mean volume of tumors developed after implantation of PIN-SCs in WT or in IL-30KO mice. Student’s t-test: p < 0.0001 versus WT mice. Results from B6 EIIa-cre mice and p28^f/f mice are not different from those obtained in WT mice (Fisher Exact Probability Test: p > 0.99). c Mean volume of tumors developed after implantation of shPIN-SCs or IL-30shPIN-SCs in WT or in IL-30KO mice. Student’s t-test: p < 0.001 (shPIN-SCs or IL-30shPIN-SCs in IL-30KO mice versus WT mice). Results from B6 EIIa-cre mice and p28^f/f mice are not different from those obtained in WT mice (Fisher Exact Probability Test: p > 0.99). d Mean volume of tumors developed after implantation of PIN-SCs, shPIN-SCs or IL-30shPIN-SCs in IL-30KO mice. ANOVA: p < 0.001. Tukey’s HSD test: p < 0.01 versus both controls. e Mean volume of tumors developed after implantation of PIN-SCs, shPIN-SCs or IL-30shPIN-SCs in WT mice. ANOVA: p < 0.01. Tukey’s HSD test: p < 0.01 versus both controls. f H&E stained sections of lung metastasis spontaneously developed in WT and in IL-30KO mice bearing shPIN-SC or IL-30shPIN-SC tumors. Magnification: × 400. Scale bars: 30 μm. g Percentage of lung metastasis spontaneously developed in WT and in IL-30KO mice bearing shPIN-SC or IL-30shPIN-SC tumors. *Fisher’s exact test: p < 0.01 versus shPIN-SC tumors in both WT and IL-30KO mice. h Histologic (H&E) and immunohistochemical features of tumors developed after sc implantation of shPIN-SCs or IL-30shPIN-SCs in WT and in IL-30KO mice. Magnification: × 400. Scale bars: 30 μm