Fig. 2
Immunopathological profile of tumors developed after sc. implantation of IL-30-silenced PIN-SCs in WT and IL-30KO mice. a Immunohistochemical features of IL-30shPIN-SC and shPIN-SC tumors developed in IL-30KO and in WT mice. Magnification: X400. Scale bars: 30 μm. The insets show double staining for CD11b (brown) and Gr-1 (red) (X630) and double staining for Foxp3 (brown) and CD4 (red) (X1000). b In shPIN-SC tumors grown in WT mice, double staining reveals that IL-30 (brown) co-localize with F4/80+ macrophages (red), whereas triple staining reveals that IL-30 (brown) also co-localizes with CD11b (blue) and Gr-1 (red), both markers for MDCs. Magnification: × 630. Scale bars: 20 μm. c Immune cell counts in IL-30shPIN-SC and control shPIN-SC tumors developed in WT and in IL-30/p28f/f mice. Results are expressed as mean ± SD of positive cells/field evaluated at X400 (0.180 mm2 field) by immunohistochemistry. ANOVA: p < 0.01. *p < 0.01, Tukey HSD Test compared with shPIN-SCs in WT or IL-30KO mice. **p < 0.01, Tukey HSD Test compared with shPIN-SCs or IL-30shPIN-SCs in WT mice. d Double immunostainings of shPIN-SC tumors developed in WT mice and IL-30shPIN-SC tumors developed in IL-30KO mice (IL-30−/−tumors) reveal a strong expression of IDO (brown), which mostly co-localize with CD11b cells (red), in IL-30+/+tumors; whereas it is scanty in IL-30−/− tumors. Magnification: × 630. Scale bars: 20 μm. e Double immunostainings of shPIN-SC tumors developed in WT mice reveal that IL-10 and TGFβ (both in red) mostly co-localize with Foxp3+ cells (brown). Magnification: X400. Scale bars: 30 μm. The inset shows the double staining for Foxp3 (brown) and IL-10 (red): X1000