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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Targeting Interleukin(IL)-30/IL-27p28 signaling in cancer stem-like cells and host environment synergistically inhibits prostate cancer growth and improves survival

Fig. 3

Immunohistochemical detection of apoptosis-related proteins and CTL cytotoxic molecules in tumors that developed after sc implantation of IL-30-silenced PIN-SCs in IL-30KO mice. a Active caspase-3 and perforin immunostainings in IL-30shPIN-SC and shPIN-SC tumors developed in IL-30KO and in WT mice. Magnification: × 630 (top images) X400 (bottom images). Scale bars: 20 μm (top images), 30 μm (bottom images). In the inset, the double staining shows perforin (red) co-localization with CD3+ cells (brown) (× 400). b Double staining of IL-30−/−tumors showed CD3+T cells (red) in close contact with caspase-3+ neoplastic cells (brown). Magnification: X1000. Scale bar: 10 μm. Double staining of IL-30−/−tumors also shows that CD4+ cells (red) mostly express TIA-1 (brown). Immunohistochemical detection of TRAIL, FasL and Fas (inset) in IL-30−/−tumors. Magnification: × 400. Scale bars: 30 μm. c Double immunofluorescent stainings of IL-30−/−tumors reveal in light blue the co-localization of TRAIL (green) and CD3+T cells (blue) and in yellow the co-localization of FasL (green) and CD4+T cells (red). Magnification: X630. Scale bars: 20 μm

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