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Table 1 Immunohistochemical features of IL-30-silenced tumors developed in WT and IL-30KO mice

From: Targeting Interleukin(IL)-30/IL-27p28 signaling in cancer stem-like cells and host environment synergistically inhibits prostate cancer growth and improves survival

 

shPIN-SC

IL-30shPIN-SC

ANOVA

p valueb

WT

IL-30KO

WT

IL-30KO

 

Microvessel densitya

17.0

±

4.2

15.5

±

4.4

7.3

±

4.0d

3.0

±

2.3c

< 0.0001

Sca-1+ (%)a

52.7

±

9.7

48.8

±

8.3

26.5

±

6.6d

20.3

±

6.9d

< 0.0001

Proliferation Index (%)a

68.9

±

8.4

65.0

±

7.5

35.8

±

6.0d

36.5

±

7.0d

< 0.0001

Apoptotic Index (%)a

5.8

±

3.0

16.9

±

4.4e,f

10.2

±

4.7

28.2

±

5.6c

< 0.0001

Caspase-3 (%)a

2.1

±

1.5

14.6

±

2.5e,f

3.3

±

1.9

21.8

±

5.0c

< 0.0001

Perforin (%)a

2.0

±

1.9

12.3

±

3.7e,f

2.8

±

2.1

30.0

±

6.3c

< 0.0001

  1. aMicrovessel density and the percentage of immunostained cells were assessed by light microscopy, at × 400 in an 85431.59 μm2 field, with Qwin image analysis software (version 2.7). Results are expressed as mean ± SD of CD31 positive microvessels per field (microvessel density), or mean percentage of positive cells/number of total cells (Sca-1, PCNA, TUNEL, caspase-3, perforin)
  2. bOne-way ANOVA for comparisons between all groups
  3. cp < 0.01 Tukey’s HSD test compared with IL-30shPIN-SC in WT mice and control tumors in WT and KO mice
  4. dp < 0.01 Tukey’s HSD test compared with control tumors in WT and KO mice
  5. ep < 0.01 Tukey’s HSD test compared with control tumors in WT mice
  6. fp < 0.01 Tukey’s HSD test compared with IL-30shPIN-SC in WT mice