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Table 1 Immunohistochemical features of IL-30-silenced tumors developed in WT and IL-30KO mice

From: Targeting Interleukin(IL)-30/IL-27p28 signaling in cancer stem-like cells and host environment synergistically inhibits prostate cancer growth and improves survival

  shPIN-SC IL-30shPIN-SC ANOVA
p valueb
WT IL-30KO WT IL-30KO  
Microvessel densitya 17.0 ± 4.2 15.5 ± 4.4 7.3 ± 4.0d 3.0 ± 2.3c < 0.0001
Sca-1+ (%)a 52.7 ± 9.7 48.8 ± 8.3 26.5 ± 6.6d 20.3 ± 6.9d < 0.0001
Proliferation Index (%)a 68.9 ± 8.4 65.0 ± 7.5 35.8 ± 6.0d 36.5 ± 7.0d < 0.0001
Apoptotic Index (%)a 5.8 ± 3.0 16.9 ± 4.4e,f 10.2 ± 4.7 28.2 ± 5.6c < 0.0001
Caspase-3 (%)a 2.1 ± 1.5 14.6 ± 2.5e,f 3.3 ± 1.9 21.8 ± 5.0c < 0.0001
Perforin (%)a 2.0 ± 1.9 12.3 ± 3.7e,f 2.8 ± 2.1 30.0 ± 6.3c < 0.0001
  1. aMicrovessel density and the percentage of immunostained cells were assessed by light microscopy, at × 400 in an 85431.59 μm2 field, with Qwin image analysis software (version 2.7). Results are expressed as mean ± SD of CD31 positive microvessels per field (microvessel density), or mean percentage of positive cells/number of total cells (Sca-1, PCNA, TUNEL, caspase-3, perforin)
  2. bOne-way ANOVA for comparisons between all groups
  3. cp < 0.01 Tukey’s HSD test compared with IL-30shPIN-SC in WT mice and control tumors in WT and KO mice
  4. dp < 0.01 Tukey’s HSD test compared with control tumors in WT and KO mice
  5. ep < 0.01 Tukey’s HSD test compared with control tumors in WT mice
  6. fp < 0.01 Tukey’s HSD test compared with IL-30shPIN-SC in WT mice