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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: miR-34a as hub of T cell regulation networks

Fig. 1

Gene Ontology subcategories of the predicted miR-34a target genes. a Enrichment of predicted miR-34a target genes in specific Gene Ontology subcategories. b Dual luciferase reporter gene assay of VAMP2, IKBKE, MYH9, MARCH8, KLRK1, CD11A, TRAFD1, CCR1, PYDC1, PRF1, PIK3R2, PIK3CD, AP1B1, ADAM10, PVR, AP2S1, BAD, ICOS, CD247, ZFP36, STX8 and SPN. HEK 293 T cells were co-transfected with miR-34a and reporter plasmids containing 3’UTRs of target genes as indicated. The luciferase activities were normalized with respect to the luciferase activity measured with empty reporter construct. The results represent the mean of four independent experiments carried out in duplicates. Columns colored in turquois represent a significant reduction of the luciferase activity with a p-value ≤0.001 (three asterisks). Columns colored in magenta represent a significant reduction of the luciferase activity with a p-value ≤0.01and ≥ 0.001 (two asterisks). Columns colored in violet represent a significant reduction of the luciferase activity with a p-value ≤0.05 and ≥ 0.01 (one asterisk). Columns colored in dark blue represent a non-significant reduction of the luciferase activity with a p-value ≥0.05. Data are represented as mean ± SEM

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