Fig. 2

Dual luciferase reporter gene assay of the mutated 3’UTRs of VAMP2, IKBKE, MYH9, MARCH8, KLRK1, CD11A, ADAM10, and PVR. HEK 293 T cells were co-transfected with the miR-34a and the wild type reporter plasmids (wt) of the respective target genes or mutated reporter plasmids (mut) of the respective target genes as indicated in the diagram. The luciferase activities were normalized with respect to the luciferase activity of the co-transfection with empty reporter construct. The results represent the mean of four independent experiments carried out in duplicates. Three asterisks correspond to a p-value ≤0.001, two asterisks to p-value ≤0.01and ≥ 0.001 and one asterisk to a p-value ≤0.05 and ≥ 0.01. A p-value ≥0.05 is indicated as n.s. (non-significant). Data are represented as mean ± SEM