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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: PD-1 silencing impairs the anti-tumor function of chimeric antigen receptor modified T cells by inhibiting proliferation activity

Fig. 7

The effect of PD-1 silencing was tested under different culture conditions and in CART-HER1 system. a and b IL-7, IL-15 and IL-21 were used instead of IL-2 for the cultivation of CART-19 cells. During the cultivation, the ZsGreen positive rates (a) and differentiation phenotypes (b) of different CAR-T cells were monitored. c and d The influence of PD-1 knockdown on CART-HER1 cells was evaluated. The ZsGreen positive rates during cultivation (c) and differentiation phenotypes on the tenth day of culture (d) in different CAR-T cells were monitored. These results indicated that the effect of PD-1 knockdown on CAR-T cells was universal. Data are plotted as individual points

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