Fig. 1

The suppressive effect of MICA-ECD on NK cell cytolytic activity. a The MICA*002 expression on the C1R-MICA*002 cell line was detected with a human NKG2D-Fc fusion protein, followed by the staining of a PE conjugated anti-Fc monoclonal antibody (red). An IL-23R-Fc protein staining was used as a specificity control (blue). b NKG2D receptor expression on primary human NK cells was detected by a PE-conjugated anti-NKG2D antibody (clone 1D11) (red), compared with isotype control antibody staining (blue). c The killing experiment of C1R-MICA*002 cells was conducted using NK cells in the presence or absence of recombinant MICA-ECD (5 μM) or no treatment. NK cell-mediated killing of the parental C1R cell line was shown as comparison. Each data point represents average of 2 technical replicates with error bar representing SEM, and the dataset is a representative of 3 independent experiments