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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: MICA immune complex formed with alpha 3 domain-specific antibody activates human NK cells in a Fc-dependent manner

Fig. 2

MICA-ECD does not down-regulate cell surface NKG2D receptor on NK cells. a Cell surface NKG2D expression on primary human NK cells was detected by an anti-NKG2D antibody (clone 26F3) (red) and isotype control antibody (blue) following the treatment of 5 μM MICA-ECD for 4 h at the indicated temperatures. b NKG2D expression on NK cells from three independent donors was detected by an anti-NKG2D antibody (clone 26F3) 24 h after treatment with 5 μM sMICA or 2 ng/mL TGF-β1. The NKG2D expression was indicated as the mean fluorescence intensity (MFI) of antibody stained cell population by flow cytometry analysis. The experiment was conducted using NK cells isolated from three independent donors (error bar representing SD), and the dataset is a representative of 2 independent experiments with p-values generated from unpaired t-test. *p > = 0.05; **p < 0.05

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