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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: MICA immune complex formed with alpha 3 domain-specific antibody activates human NK cells in a Fc-dependent manner

Fig. 3

Suppressive effect of MICA-ECD on NK cytolytic activity is reversed by an anti-MICA antibody. a The binding of NKG2D-Fc fusion protein to the C1R-MICA*002 cell line was conducted in the presence of increasing amount of an anti-MICA antibody clone AMO1 (MICA α1α2-specific) or the anti-MICA/B mAb clone 6E1. The normalized mean fluorescence intensity (MFI, %) of NKG2D-Fc fusion protein binding is shown, each data point represents average of 2 technical replicates with error bar representing SEM, and the dataset is a representative of 2 independent experiments. b C1R-MICA*002 cell line killing was conducted side by side with C1R cells using human NK cells. NK cells were pretreated with MICA-ECD, MICA-ECD plus anti-MICA/B clone 6E1 or no treatment for MICA*002 cell line killing, and NK cell killing of parental C1R cell line was used for comparison. Each data point represents average of 2 technical replicates, and the dataset is a representative of 3 independent experiments with p-values generated from unpaired t-test. *p > = 0.05; **p < 0.05

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