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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Late-differentiated effector neoantigen-specific CD8+ T cells are enriched in peripheral blood of non-small cell lung carcinoma patients responding to atezolizumab treatment

Fig. 3

Neoantigen-specific T cells in atezolizumab responder patients show a more differentiated effector phenotype. a Heatmap representing frequency of antigen-specific CD8+ T cells positive for all phenotypic markers analyzed. Results for all neoantigen-specific and virus-specific CD8+ T cells detected in individual patients are shown, grouped by responders and non-responders. Markers are ordered based on unsupervised hierarchical clustering. Numbers in brackets correspond to unique neoantigens detected in each patient. b The first two components obtained from PCA of percentages of neoantigen-specific T cells for each marker are plotted for each hit (left). Boxplots show the trends toward a higher number of neoantigen-specific T cells positive for CD27, CD28, CD127, and CCR7 in the non-responder group and 2B4, KLRG-1, CD57, CD161, TIGIT, and CD25 in the responder group, respectively (Wilcoxon signed rank test). c Biaxial dot plots showing an example of neoantigen-specific T cells displaying an activated phenotype with co-expression of PD-1 and CD39. t-SNE plots are based on the expression of all phenotypic markers. Relative expression levels of CCR7 and CD45RO are shown. Data shown from Patient 4 (red, neoantigen-specific T cells; blue, EBV-specific T cells; grey, bulk CD8+ T cells)

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