Fig. 4

α-GalCer-treatment increase the frequency of IFN-γ-producing CD8⁺ T cells and Th1 cells. Naïve C57BL6 mice were given s.c. injection of B16F10 cells (1 X 10⁶ cells/mouse) and also injected anti-NK1.1 mAb and treated with α-GalCer as in Fig. 3a. a At day 13, IFN-γ⁺ production in the splenic and intratumoral CD8⁺ T cells was analyzed after gating on lymphocytic gate followed by singlet populations. n = 4–5 mice/group. b At day 13, CD4⁺IFN-γ⁺ T cells were analyzed after gating on CD4⁺ cells. n = 4–5 mice/group. The bar represents s.e.m., and each dot represents data from an individual mouse. Student’s t-test. ** p < 0.01, ns, not significant