Fig. 4

CPR treatment enhances intratumoral CD8⁺ T cell infiltration and activation. Established mEER tumors were treated with components of the CPR regimen and harvested on day 23 of treatment, or day 23 and day 37 for the full CPR regimen, and tumor lymphocyte infiltrates were analyzed using quantitative multiplex immunofluorescence. a Representative multiplex images of mEER tumors showing DAPI (nuclei, dark blue), EpCAM (tumor, red), and CD8 (CD8⁺ T cells, cyan). Zoomed middle insert shows a representative T cell from a control and day 37 CPR treated tumors with DAPI/EpCAM/CD8 stain on left and DAPI/Granzyme B (activated T cell marker, green) on right. b Pie-chart showing T cell subset densities as a fraction of the whole T cell tumor infiltrate by treatment group. Pie area corresponds to the total T cell density per treatment group. c Log2 fold-change of lymphocyte subset densities (counts per total nucleated cells) in CPR tumors vs control tumors statistically comparing day 23 and day 37 of CPR treatment (Tukey’s multiple comparison). For all samples N = 1 and cellular densities were averaged across 5 images per tumor with n = 3 per group. ***p < 0.001; ****p < 0.0001