Fig. 1

PD-L1-blocking treatment induces delay of MC-38 tumor growth. (a) Schematic of CyTOF mass cytometry experiment investigating the effect of PD-L1 antibody treatment on the TME. Mice were challenged with MC-38 tumor cells and subsequently tumor-bearing mice were treated either with PBS (n = 16 mice) or PD-L1 blocking antibodies (n = 16 mice). Tumors were isolated and analyzed by mass cytometry (CyTOF). Cluster identification was performed with HSNE and downstream analysis was done with Cytofast. (b) Tumor growth curves of individual mice in the control group (mock injected with PBS, blue lines) and PD-L1-treated group (red lines). (c) Frequency of CD45⁺ PD-L1⁺ cells in the TME 8 days after therapy starts displayed on a per-mouse basis with mean ± SEM. (d) Overview of the immune cell composition in the TME shown in percentage of cells on a per-mouse basis with mean ± SEM (n = 16 mice per group)