Fig. 5

Quantification and cytotoxic capacity of the T_AI cells in the TME (a) Average percentage (and SEM) of T_AI cells within the CD8⁺ (left panel) and CD4⁺ (right panel) T-cell compartment at day 3 and 8 post PD-L1 blockade therapy in MC-38 tumor challenged mice. (b) Granzyme B expression of CD8⁺ T cell subsets at day 8 post PD-L1 treatment in MC-38 tumor bearing mice. The grey shaded histograms represent the CD39⁻ PD-1^+/− CD8⁺ T cells and the red shaded histograms depict the CD39⁺ PD-1⁺ CD8⁺ T_AI cells of individual mice. (c) The percentage of granzyme B⁺ cells among the CD39⁺ PD-1⁺ CD8⁺ T_AI cells after 8 days of PD-L1 treatment in the MC-38 tumor model compared to the CD39⁻ PD-1^+/− CD8 T cells