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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Colorectal cancer cell-derived CCL20 recruits regulatory T cells to promote chemoresistance via FOXO1/CEBPB/NF-κB signaling

Fig. 4

Tregs enhance the chemoresistance of CRC to 5-FU. a Before and after treatment with the supernatants of Tregs, cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM) was analyzed by the CCK8 assay. b With different concentrations of Treg supernatants (supernatant frequency of Treg/tumor cell = 1:1, 1:2 and 1:3), cell viability of SW620 cells treated with 5-FU (10, 20, and 50 μM) was analyzed by the CCK8 assay. c Relative expression of resistance-related genes in SW620 and DLD1 cells was analyzed by qPCR. d Relative expression of CSC-related genes in SW620 and DLD1 cells before and after treatment with Treg supernatants was analyzed by qPCR. e The relationship between FOXP3 and resistance-related genes from TCGA dataset was analyzed. *P < 0.05, **P < 0.01

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