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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Colorectal cancer cell-derived CCL20 recruits regulatory T cells to promote chemoresistance via FOXO1/CEBPB/NF-κB signaling

Fig. 5

FOXO1/CEBPB/NF-κB signaling is required for CCL20 upregulation mediated by 5-FU. a Screening graph showing the relationship between RELA and other signaling pathways using cBioPortal for Cancer Genomics. b Relative expression of related genes predicted in a was analyzed from TCGA dataset. c Relative expression of FOXO1 and CEBPB in SW620 cells before and after treatment with 5-FU was analyzed by qPCR. d Relative expression of FOXO1 and CEBPB in stable FOXO1/CEBPB knockdown SW620 cells was analyzed by qPCR. e Relative expression of CEBPB and CCL20 in shFOXO1 SW620 cells treated with 5-FU was analyzed by qPCR. f Relative expression of FOXO1 and CCL20 in shCEBPB SW620 cells treated with 5-FU was analyzed by qPCR. FOXO1/CEBPB/NF-κB signaling activation in SW620 cells after treatment with 5-FU at different times (5, 20, and 60 min) (g) and with different doses (0.25, 5, and 10 μg/ml) (h). FOXO1, CEBPB, and phospho-P65 protein levels were assessed by western blotting. FOXO1/CEBPB/NF-κB signaling activation in shFOXO1 (i) and shCEBPB (j) SW620 cells treated with 5-FU was analyzed by western blotting. *P < 0.05, **P < 0.01, ***P < 0.001

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