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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Indocyanine green and poly I:C containing thermo-responsive liposomes used in immune-photothermal therapy prevent cancer growth and metastasis

Fig. 4

piTRL treatment followed by laser irradiation promoted activation in tumor-draining lymph node (tdLN). CT-26 tumor-bearing mice were i.t. injected with PBS, TRL, iTRL, piTRL or poly I:C, and treated with or without laser irradiation for 5 min. tdLN were harvested 24 h after laser irradiation. a Definition of DC population in tdLN was shown. Lineage markers included CD3, Thy1.1, B220, Gr-1, CD49b, and TER-119. LineageCD11c+ DCs were further divided as CD8α+ and CD8α DCs. b Frequency of tdLN DCs is shown. c Mean absolute number of LineageCD11c+ cells in tdLN is shown, ** p < 0.01. d Mean fluorescence intensity (MFI) of co-stimulatory molecules and MHC class I and II in gated CD8α+ and CD8α DCs in tdLN was analyzed using flow cytometry. e Levels of IL-6, IL-12p40 and TNF-α mRNA in tdLN. All data are representative of the average of the analyses of six independent samples (i.e., three samples per experiment, two independent experiments)

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