Fig. 6

Induction of cancer Ag-specific immune responses by piTRL. BALB/c and C57BL/6 mice were subcutaneously injected with cancer cells (1st transplanted tumor) and treated by the liposomes as shown in Fig. 5. a and b Spleens were harvested on day 10 of the 2nd transplantation of tumor. The splenocytes were stimulated with a CT-26 or b B16 self-Ag for 24 h and IFN-γ production was measured by ELISPOT. ** p < 0.01. c and d Specific lysis of cells was analyzed on day 10 of the 2nd transplantation of tumor in mice by transferring cancer Ag- or control peptide-coated splenocytes. ** p < 0.01. e and f B16 tumors in C57BL/6 mice were treated with piTRL and laser irradiation as indicated in Fig. 5. On day 25 of the 1st transplant of B16 cells, the mice received e depletion abs (anti-CD4 and anti-CD8 abs) or f blockade abs (anti-CD80 and anti-CD86 abs). The curves show survival rates of mice (n = 5 for each group)