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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Discovery of low-molecular weight anti-PD-L1 peptides for cancer immunotherapy

Fig. 5

3D spheroid penetration of the CLP002 peptide and anti-PD-L1 antibody. 3D tumor spheroids of MDA-MB-231 cells were generated to compare the tumor penetration capability of the CLP002 peptide and the anti-PD-L1 antibody (BioXcell, 29E.2A3). Cy5-labeled peptide and antibody were incubated with the tumor spheroids (~ 700 μm in diameter) for 2 and 6 h, followed by confocal microscopy analysis to evaluate tumor penetration. a Representative Z-stacked confocal images of the spheroids with a z-step of 50 μm. The scale bar represents 200 μm. b The depth of penetration is quantified by mean fluorescence intensity. Results are represented as the mean ± SD (n = 3)

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