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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Isolation of T cell receptor specifically reactive with autologous tumour cells from tumour-infiltrating lymphocytes and construction of T cell receptor engineered T cells for esophageal squamous cell carcinoma

Fig. 1

Phenotype and functional screening of different tumor infiltrating lymphocytes (TILs) fragments. a Flow cytometry analysis revealed percentages of CD4+ and CD8+ T cells from TIL-F1 to TIL-F4. b CD4/CD8 ratio. c The percentages of PD-1+T cells in four TIL fragments. d Comparision of PD-1 expression. e Comparison of memory-phenotype T cells. f IFN-γ ELISPOT analysis of all four TIL fragments cocultured with autologous tumor cells (ATCs). TILs with no targets are negative controls. Medium well is the blank negative control and OKT-3 well is the positive control. Column histogram summarized the number of positive spots. g IFN-γ ELISA measurement of all four TIL fragments cocultured with ATCs. T cells with no targets are negative controls. The results shown are representative of independent experiment done with repeated 3 times

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