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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Isolation of T cell receptor specifically reactive with autologous tumour cells from tumour-infiltrating lymphocytes and construction of T cell receptor engineered T cells for esophageal squamous cell carcinoma

Fig. 2

Flowchart for isolation of TCRs from CD137 positive TIL-F1 and function verification of corresponding TCR-Ts. TIL-F1 was stimulated with ATCs for twice, and single CD8+ CD137+ TIL-F1 before and after stimulation (namely PRE and POST) were both sorted into 96-well plates and were amplified using single-cell RT-PCR to obtain their TCRs. Subsequently, the most dominant TCR in CD8+ CD137+ TIL-F1 before and after stimulation was cloned into lentiviral vector and introduced into donor peripheral blood lymphocytes (PBLs) to generate tumor-reactive T cell receptors-engineered T cells (TCR-Ts). Finally, we evaluated whether this TCR-Ts could specifically identify and recognize ATCs in vivo and in vitro

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