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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: High numbers of activated helper T cells are associated with better clinical outcome in early stage vulvar cancer, irrespective of HPV or p53 status

Fig. 4

Tumor-infiltrating lymphocytes produce Th1 and Th2 cytokines upon mitogenic stimulation. In vitro expanded T cells from VSCC were analyzed for their cytokine production following mitogenic stimulation with 0.5 μg/ml PHA for 4 days, after which supernatants were harvested and analyzed by cytometric bead array (CBA) to determine the production of IFN-γ, TNF-α, IL-10, IL-5, IL-4 and IL-2 in pg/mL. Mean (± SEM) cytokine production is shown for 14 HPVnegVSCC

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