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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: High numbers of activated helper T cells are associated with better clinical outcome in early stage vulvar cancer, irrespective of HPV or p53 status

Fig. 5

HPVnegVSCC are infiltrated with highly activated CD4+ and CD8+ effector/memory T cells. PBMC of healthy controls (n = 11) as well as PBMC (n = 29) and freshly dissociated tumor-derived TIL (n = 12) of HPVnegVSCC patients were analyzed by 13-parameter flow cytometry analysis. a Hierarchical Stochastical Neighbor Embedding (HSNE) clusters (left) and density plots (right) visualizing the high-dimensional flow cytometry data in two dimensions for the collective total CD3+ T cells for indicated groups. The identified cell subsets are identified in the cluster plots by the different colors. b CITRUS automatic discovery of stratifying biological signatures within tumor and blood samples visualizes 10 distinctive populations of CD8+ and CD4+ T cells the total CD3+ immune population. Every cell population represented by a node is divided on basis of median level of expression of a differently expressed marker into two new nodes (cellular subsets) going from the center (all cells) to the periphery of the plot. c The distribution of CD4+ and CD8+ T-cell frequencies (mean ± SEM) within the total CD3+ T cell population is depicted for healthy control and VSCC PBMC and tumors. d Scatter plots with bars displaying frequencies of CD8+ (# 1 to 5; top panel) and CD4+ (# 6 to 10; bottom panel) T cell populations are given as % of CD8+ and CD4+ cells. (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001)

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