Fig. 4

Immune phenotype associated with development of clinical responses and autoimmunity, and the effect of steroids. a Unsupervised hierarchical clustering of indicated immune populations in PBMC prior to treatment with avelumab. Higher levels of expression are indicated in red and lower levels of expression are indicated in blue. Patient response (R, responders; NR, non-responders) and development of immune-related adverse event (irAE) are indicated. b Diversity of TCR repertoire in PBMC of patients prior to therapy with avelumab. c Diversity of the TCR repertoire in PBMC of patients pre- and post-steroid treatment. TCR diversity was measured by the metric of repertoire size; values in panels B and C indicate the number of individual clonotypes comprising the top 25th percentile by ranked molecule count after sorting by abundance. The day (D) PBMC were assessed for TCR diversity pre- and post-steroids is indicated