Fig. 3

Intratumoral administration is required for MEDI9197 anti-tumor effects and is efficacious in diverse syngeneic models. a Anti-tumor effects was measured in the B16-OVA tumor model following IT injection or SC dosing, away from the tumor on the opposite flank. C57BL/6 J albino mice were implanted SC B16-OVA tumor cells the left flank on Day 0 (20 mice/group). On Days 8 and 15, mice were dosed IT with MEDI9197 [20 μg/50 μL], Resiquimod [20 μg/50 μL], or Vehicle (sesame oil/EtOH, 50 μL]). Some mice were dosed SC with MEDI9197 on the opposite side of the implanted tumor (right flank). Naïve mice were untreated. Mice were euthanized when tumor size equaled or exceeded 2500 mm³. The Kaplan-Meier plot shows survival for each group up to Day 90. ****P < 0.0005; MEDI9197 IT group (solid red line) compared with each of the other groups using the Log-rank test. b and c. Tumor volume was assessed in 4 T1, B16-F10 AP3 CAG luc2, MC38 single-flank mouse models following IT administration of MEDI9197 (20 μg) or Vehicle. MEDI9197 and Vehicle were dosed weekly for 2 or 3 doses as described in the figure. b Spider plots represent tumor volume of individual mice. Vehicle-treated mice are represented with black lines, and MEDI9197-treated mice are represented with red lines. c B16-F10 CAG luc2 tumors (9 mice/group) were treated with either 0.4 or 20 μg MEDI9197 weekly on days 5, 12, and 19. D-Luciferin was I.P. injected 15 min prior to imaging bioluminescence on the IVIS100. Data represents the total counts +/− SEM. Statistical analysis was performed by Dunnet’s multiple comparisons test. ***p = 0.0004, NS p = 0.1175. Representative images from day 20 post implantation are shown