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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Targeting interferon signaling and CTLA-4 enhance the therapeutic efficacy of anti-PD-1 immunotherapy in preclinical model of HPV+ oral cancer

Fig. 2

Differential infiltration of T cells between oral and subcutaneous mEER tumors. Tumor-infiltrating leukocytes were isolated at day 15 after tumor implantation from mice bearing flank or tongue mEER tumors and analyzed by flow cytometry. Figure shows percentage of different leukocyte subsets among total live lymphocytes (a) and frequencies of PD-1 expressing CD8+ T cells (b). Results represent pooled data from two separate experiments (n = 8–10). Statistical significance was calculated using Two-way ANOVA **p < 0.005, ****p < 0.00005. Flank and Tongue implanted mEER tumors (n = 3–6) were analyzed for PD-L1 expression by real-time qPCR (c) and fluorescence immunohistochemistry (d, e). Representative IHC images (d) and quantification of PD-L1+ cells (e) are shown. **p = 0.0053, Student’s t-test

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