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Table 1 Inhibitory effect of H5B14-based ADCs on cell viability from a panel of cancer cell lines

From: Therapeutic efficacy of a novel humanized antibody-drug conjugate recognizing plexin-semaphorin-integrin domain in the RON receptor for targeted cancer therapy

Cancel cell lines IC50 values of H5B14-MMAE and H5B14-DCM in cell viability (μg/ml)a
Zt/g4-MMAE Zt/g4-DCM PCM5B14-MMAE PCM5B14-DCM H5B14-MMAE H5B14-DCM
MCF-7 > 100 > 100 > 100 > 100 > 100 > 100
DU4475 2.28 3.96 2.75 2.46 3.04 1.81
MDA-MB-231 6.89 3.48 5.38 5.98 6.25 5.30
T-47D 1.77 1.22 3.11 1.55 2.35 1.63
LoVo > 100 > 100 > 100 > 100 > 100 > 100
HCT-116 2.21 0.94 3.31 1.58 2.83 1.37
HT-29 1.65 1.65 3.15 2.97 2.88 2.09
SW-620 2.97 2.65 5.44 1.92 3.35 2.95
H1993 > 100 83.89 > 100 > 100 > 100 74.07
H2228 3.86 4.28 5.63 4.65 4.49 3.64
H358 3.85 2.96 2.77 3.51 1.46 1.87
Panc-1 > 100 > 100 > 100 > 100 > 100 > 100
BxPC-3 3.04 3.89 3.25 5.25 2.68 3.02
FG 2.14 0.74 1.13 0.96 1.34 1.15
L3.6pl 1.83 2.41 2.47 4.48 3.03 1.88
Average 2.95 ± 1.52 2.61 ± 1.36 3.49 ± 1.41 3.21 ± 1.68 3.06 ± 1.36 2.43 ± 1.22
  1. aindividual cancer cell lines at ~ 8000 cells per well were cultured in triplicate in the presence of absence of different amounts of H5B14-MMAE or H5B14-DCM for 72 h. Zt/g4-based ADCs and PCM5B14-based ADCS were used for comparison. The MTS assay was used to determine the cell viability. The IC50 values for individual cell lines were calculated using the GraphPad Prism 7 software as previously described [1]