Fig. 3

Niclosamide suppress p-STAT3 and PD-L1 expression in a dose- and time-dependent manner. a Representative changes in membrane PD-L1 expression, evaluated by flow cytometry analysis on H460 cells treated with the niclosamide. b-d, h-j H460 and A549 cells were treated with different concentrations of niclosamide for 48 h, p-STAT3, STAT3 and PD-L1 expression was measured by Western blot. e-g, k-m H460 and A549 cells were treated with 2 μM niclosamide for different time intervals, p-STAT3, STAT3 and PD-L1 expression was measured by Western blot. * P < 0.05, ** P < 0.01, *** P < 0.001. Error bars represent SD of three independent experiments