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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Niclosamide, an antihelmintic drug, enhances efficacy of PD-1/PD-L1 immune checkpoint blockade in non-small cell lung cancer

Fig. 5

Knockdown of STAT3 combined with PD-L1 antibody exhibits enhanced anti-cancer immunity. a T cell cytotoxicity test by colony formation assay. The survival of H460 and H1299 cells with or without genetic downregulation of STAT3, treated with PD-L1 Ab or not, and co-cultured with PBMCs in 24-well plates for 4 days was estimated. Colonies were visualized by Giemsa staining. Survival relative to control is shown. b-e The effect of STAT3 knockdown H460 and H1299 cells as well as control cells combined with PD-L1 Ab or not on CD4+ and CD8+ populations among CD3+ T cells. Results are presented as means ± S.D. of a representative experiment performed in triplicate. f-g Intracellular cytokine staining of granzyme B in T cell-mediated tumor cell killing assay in STAT3 knockdown H460 and H1299 cells as well as control cells combined with PD-L1 Ab or not. h-i Intracellular cytokine staining of TNF-α in T cell-mediated tumor cell killing assay in STAT3 knockdown H460 and H1299 cells as well as control cells combined with PD-L1 Ab or not. Results are presented as means ± S.D. of a representative experiment performed in triplicate. * P < 0.05, ** P < 0.01, *** P < 0.001

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