Fig. 1
From: TLR9 acts as a sensor for tumor-released DNA to modulate anti-tumor immunity after chemotherapy
Effect of chemotherapy induced tumor DNA release on the anti-tumor immune response following chemotherapy. a) Quantification of DNA released from tumor cells in vivo with or without cisplatin (n = 5). b-e TC-1 tumor-bearing C57BL/6 mice were treated with cisplatin intraperitoneally, together with intratumoral injection of unlabeled (c-d) or FITC-labeled (e) E7 peptide. Mice were then administered with either DNase I or PBS. b Schematic diagram. c Line-graph depicting tumor growth kinetics in DNase I-treated compared to PBS-treated mice (n = 5). d PBMCs were collected from mice, stained with E7-Db tetramer, and examined by flow cytometry. Left: Representative flow cytometry depicting the frequency of E7-specific CTLs. Right: Bar graph quantification (n = 5). e Draining lymph nodes were processed into single cells and stained for CD11c. Left: Representative flow cytometry depicting the frequency of E7-loaded tumor DCs in the draining lymph nodes. Right: Bar graph quantification (n = 5). f-h CT26 tumor-bearing BALB/c mice were treated with cisplatin intraperitoneally, together with direct AH1-A5 peptide injection into the tumor. Mice were co-treated with either DNase I or PBS. f Line-graph depicting tumor growth kinetics (n = 5). g Kaplan-Meier survival analysis of mice (n = 5). h PBMCs were collected, pulsed ex vivo with AH1-A5 peptide, and co-stained the next day for CD8 and IFN-γ. Left: Representative flow cytometry depicting the number of systemic AH1-A5-specific CTLs. Right: Bar graph quantification (n = 5). Significance determined by student’s t test (a, c-e, & h) or ANOVA (f-g). Data are represented as mean ± SD. *P < 0.01