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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Extracellular NK histones promote immune cell anti-tumor activity by inducing cell clusters through binding to CD138 receptor

Fig. 5

NK-histones specifically bind to CD138 on MM cell surface. a. CD138 expression in MM and non-MM K562 cells. b: MM cells incubated with either H2AZ or H4 for 1 h and stained for CD138 and CD56. c-d: confocal microscopy images (c) and colocalization analysis (d) of CD138 and H2AZ after addition of recombinant H2AZ to ARP1 MM cells. e. CB-NK overexpressing H2AZ-GFP co-cultured with MM cells showing co-localization of H2AZ-GFP and CD138. f. MM cells and CB-NK (either ctrl or with knock-down of histones H2AZ, H4 and H1.5) were co-cultured for 3 h with MM cells and CD138 expression was analyzed (g). h. SIPs from co-cultures in f were added into MM cells alone for 40 min to analyze CD138 and CD56 expression. *p < 0.05

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