Fig. 3

Evolution of Tumor Microenvironment. Samples collected over time. Images at 100X (a) of tumor infiltrating immune cells (ICs), PDL1 in tumor cells and tumor infiltrating immune cells, CD8 T cells and CD163 macrophages were assessed by hematoxylin& eosin or immunostaining. b Quantification of the parameters in (A) are displayed as a percentage of tumor area and evaluated over time with respect to atezolizumab first exposure. c RNA-based signatures associated with T cells, regulatory T cells, CD8 effector T cells, NK cells, B cells, macrophages, immune checkpoints, cancer associated fibroblasts, cytolytic activity, antigen processing, angiogenesis, and proliferation were derived from RNA-Seq and plotted as PC1 scores and displayed over time. As reference, the aggregated value for the samples from the TNBC cohort in the PCD4989g study (PCD, all) is displayed as box plots representing median, 25th and 75th percentiles and the vertical bars represent range (maximum and minimum). d TNBC subtype classifiers were derived from RNA-Seq for each sample. Heatmap denotes relative RNA expression of genes involved in the subtype classifiers in the analyzed samples. BLIA and BLIS prob.: probability that the samples are BLIA or BLIS