Fig. 6
Long term anti-MM efficacy of IL-15 activated NK cells after CXCR3 blockade. Activated NK cells (6 × 105) were i.v. transferred into MM-bearing mice 3 weeks after 5TGM1 cell injection with control hamster IgG or anti-CXCR3 blocking mAb (2 doses of 250 μg). Tumor growth was determined by FACS analysis of CD138+ (tumor) cells among BM cells 7 days after transfer. Lower graph: Activated NK cells were labeled with 2.5 μM CFSE and adoptively transferred in tumor-bearing mice and donor BM NK cell number was determined 7 days later by FACS analysis of CD3-NK1.1+ cells within donor CFSE+ cells and normalized with the number of input cells (% of input cells). Average number ± SEM of 2 independent experiments (n = 4 mice/group) is shown. Student t test was performed to compare control Ig versus anti-CXCR3 treated mice. **p < 0.01