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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Distinct tumor microenvironments of lytic and blastic bone metastases in prostate cancer patients

Fig. 2

Gene expression from decalcified FFPE prostate cancer in bone. a 16 FFPE derived RNA samples (6 lytic and 10 blastic) were analyzed on an Agilent Tape Station for concentration and integrity to produce RNA Integrity Scores (RIN). b 3 lytic and 4 blastic samples contained sufficient RNA (25-100‚ÄČng) to endure adequate probe coverage of the NanoString Human Immune Oncology 360 gene expression panel. Differential expression revealed a list of significantly upregulated (moving right) and downregulated genes (moving left) in lytic prostate cancer metastases compared to blastic types. c Blastic samples were enriched for JAK-STAT pathway genes while (d) Lytic samples were enriched for PI3K-AKT gene expression. e, f Lytic samples based on gene expression demonstrate increased immune cell populations relative to blastic samples. Graphs created using Advanced Analysis module from NanoString nSolver application

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