Fig. 3
From: Distinct tumor microenvironments of lytic and blastic bone metastases in prostate cancer patients
Digital Spatial Profiling of the microenvironment for prostate cancers in bone. a Graphic depiction of the process for Digital Spatial Profiling (DSP) whereby tissue sections are selected for Regions of Interest (ROI) and profiled for antibody labeled detection. b Fluorescence image of ROI selection of lytic and blastic prostate cancer in bone where tumor (PanCK-cyan) ROIs are 600 μm circles and T cell (CD3-magenta) and Macrophage (CD68-green) ROIs are 300 μm. c Tumor ROIs stained cyan with Pan-Cytokeratin (PanCK) were measured for significant antibody presence in 3 patients with at least 4 ROIs per patient. d Macrophage ROIs were selected from regions excluding tumor cells and osteoclasts stained with CD68 antibody. e T Cell ROIs were selected from regions with high density T cell staining by CD3 antibody. * indicates statistical significance p = < 0.05 and ** indicates p = < 0.001 by Mann-Whitney test. Lytic samples are colored in red and blastic samples are colored in blue for all graphs