Fig. 5

CXCR3 mediates the attraction of CD8⁺ CTLs and Treg cells, and inhibits the CRC development. a FACS-purified cells (as depicted Fig. 1c) from colonic tumors of Cdx2-Cre⁺/Apc^F/WT mice were subjected to q-RT-PCR analysis. b-e Bone marrow cells were harvested from WT and Cxcr3^−/− mice, and transferred into lethally irradiated 6–8-week-old Cdx2-Cre⁺/Apc^F/WT mice. Recipient mice were sacrificed at 5 months of age, and their colorectal tumors were used for flow cytometry (b and c, n = 7), q-RT-PCR (d, n = 16), and tumor statistics (e, n = 9). Cells shown in c were gated as live/CD45⁺. Data represent means ± S.E.M. *p < 0.05 in Students’ t test