Fig. 6
Blockade of IL-17 signaling in CRC results in enhanced immune checkpoint signaling. a q-RT-PCR analysis of normal colon tissue and colorectal tumors from Cdx2-Cre+/ApcF/+ mice that harbor heterozygous or null alleles of Il17ra gene (n = 12). b 4-month-old Cdx2-Cre+/ApcF/+ mice received i.p. injection of 100 μg isotype or anti-IL-17A antibodies every 3 days for 1 month. Mice were sacrificed for q-RT-PCR analysis (n = 9). c Cdx2-Cre-ERT2+/ApcF/F mice that were Il17ra+/− or Il17ra−/− were sacrificed 5 weeks after tamoxifen-induced Apc ablation, and their MLN and tumors were subjected to q-RT-PCR analysis (n = 5 for MLN, 11 for tumor). d Cdx2-Cre-ERT2+/ApcF/F mice that were Il17ra−/− or Il17ra+/− were sacrificed 5 weeks after tamoxifen-induced Apc ablation, and their MLN and tumors were subjected to flow cytometry analysis. n = 5. Data represent means ± S.E.M. *p < 0.05 in Students’ t test