Fig. 7
Blockade of CTLA-4 induced the expression of IL-17 in CRC. a, b 5-month-old Cdx2-Cre+/ApcF/+ mice received i.p. injection of 100 μg isotype or blocking antibodies against CTLA-4 (a, n = 24) or PD-1 (b, n = 8) every 3 days for 2 weeks, and were sacrificed for q-RT-PCR analysis. Data represent means ± S.E.M. *p < 0.05 in Students’ t test. c: IL-17 signals directly to tumor cells in CRC to inhibit the production of CXCL9 and CXCL10. These two chemokines are required for the recruitment of CD8+ CTLs and Tregs, which inhibits CRC development by targeting cancer cells and dampening tumor-promoting inflammation, respectively