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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Immunogenicity of prostate cancer is augmented by BET bromodomain inhibition

Fig. 5

BET Bromodomain Inhibition Augments Antitumor Immunity and Increases Tumor Infiltration. a Volume of MycCap tumors treated as indicated, rx initiated d28 post-implatation. Each line represents an individual tumor. Arrows indicate start of treatment on d26. N = 10/group, repeated × 2. b Summary data of mean tumor growth for A. N = 10/group, repeated × 2. Error bars represent S.E.M. Arrows indicate start of treatment on d26. c Mean survival of animals with MycCap tumors in indicated treatment groups. N = 10/ group, repeated × 2. Lines indicate mean survival. Error bars represent S.E.M. d Survival of animals with MycCap tumors in indicated treatment groups. N = 10/group, repeated × 2. Lines indicate percent survival. Significance calculated using Log-rank (Mantel-Cox) test. **p = 0.0046 for Vehicle vs. JQ1 + aCTLA-4, *p = 0.0278 for Vehicle vs. aCTLA-4. No other comparisons between growth curves were significant. e Live CD45+TCRb+CD4CD8+ (CD8) cells /mg tumor in MycCap tumors from animals treated as indicated. N = 10/group, repeated × 2. f Live CD45+TCRb+CD4+CD8FoxP3+ (Treg) / mg tumor in MycCap tumors from animals in indicated treatment groups. N = 10/group, repeated × 2. g Ratio of live CD45+TCRb+CD4CD8+ (CD8) cells to CD45+TCRb+CD4+CD8FoxP3+ (Treg) cells in MycCap tumors from treatment groups indicated. N = 10/group, repeated × 2. h TNFα and Granzyme B secretion from stimulated live CD45+TCRb+CD4CD8+ (CD8) cells in MycCap tumors from mice in treatment groups as indicated. Gates were set based on non-stimulated CD8 controls. i Summary of mean fluorescence intensity (MFI) of Granzyme B staining in stimulated live CD45+TCRb+CD4CD8+ (CD8) cells in MycCap tumors from mice in treatment groups as indicated. N = 10 mice/group, repeated × 2. j Summary of mean fluorescence intensity (MFI) of TNFα staining in stimulated live CD45+TCRb+CD4CD8+ (CD8) cells in MycCap tumors from mice in treatment groups as indicated. N = 10 mice/group, repeated × 2. * p < 0.05, **p < 0.01, *** p < 0.001, ****p < 0.0001. P values were calculated through one-way ANOVA. Error bars shown represent S.E.M

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