Fig. 4

SPEAR T-cell therapy does not affect antigen expression or presentation. a Representative IHC images of NY-ESO-1 expression at each of the time points evaluated. Scale bar = 100 μm. b NY-ESO-1 protein expression H-scores as determined by IHC in pre- and post-infusion biopsies from all patients where a minimum of one post-infusion biopsy was evaluable (N = 15). Where > 1 biopsy per timepoint was evaluated, average H-score is shown. Mann-Whitney statistical test was used to evaluate changes between pre- and post-infusion time points. Tumor-associated antigen (c) and antigen-processing machinery (d). RNA expression shown as normalized counts as determined by the NanoString assay performed on pre- and post-infusion biopsies. Where more than one biopsy was collected and tested separately, points show the mean. Box-plots depict the median, along with first and third quartiles