Fig. 5

Adoptively transferred NY-ESO-1 SPEAR T cells maintain functionality long after infusion. a Representative fields for detection of negative control RNA (DapB), positive control RNA (PPIB, POLR2A), and CD3 or NY-ESO-1^c259TCR RNA by RNAish in one patient’s tumor collected over 2 years post-infusion. b A375 target killing shown as green object count as determined by the Incucyte killing assay performed on flow sorted CD3⁺CD8⁺dextramer⁺ T cells (red line) and CD3⁺CD8⁺dextramer⁻ T cells (grey line) from a patient’s PBMCs collected 12 months post-infusion and on A375 alone (blue line)