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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Antibody drug conjugates against the receptor for advanced glycation end products (RAGE), a novel therapeutic target in endometrial cancer

Fig. 3

Conjugated RBGO1 antibody is rapidly internalized and trafficked to the endomsomal compartment. HEC1A, Ishikawa, HEC50 or HEC1B endometrial cancer cells were treated with control medium or medium containing RBGO1 antibody conjugated to a pH sensitive dye for 30 min, 1 h or 4 h (a). Images were acquired on a Zeiss LSM 710 confocal microscope and analyzed using the Zen 2012 image analysis software. The quantity of internalized antibody was determined using Image J software as a function of cell area (G). Histogram data are expressed as mean (SD) from 3 independent experiments (b). Data were analyzed by ANOVA and Dunnett’s multiple comparison test. Values differ from 30 min, **p < 0.01, ***p < 0.001, ****p < 0.0001

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