Fig. 5

ADU-1805 is anticipated to have a favorable safety profile over CD47-targeting agents. a In contrast to anti-CD47 (AB6.12-IgG4PE), ADU-1805 does not bind to human platelets and RBCs, consistent with its binding specificities. (Mean; representative of n = 6 is shown). b ADU-1805 does not trigger hemagglutination. Anti-CD47 clone B6H12 and phytohemagglutinin (PHA-P) serve as positive control. (Mean; representative of n = 12 is shown). c ADU-1805 does not alter T-cell responses in an allogeneic MLR whereas anti-CD47 inhibits T-cell activation. The allogeneic immune reaction, when lymphocytes of two different donors are combined, results in T-cell activation. The resulting proliferation and/or production of cytokines were analyzed 5 days after start of culture. d Inhibition of T-cell activation by anti-CD47 coincides with a depletion of CD4+ T-cells. (c, d: Mean ± SD; representative of n = 3 donor combinations is shown). Data were analyzed by unpaired two-sided Student’s t-test. * indicate statistical differences compared to the respective isotype control group: *p < 0.05, ***p < 0.001, ****p < 0.0001; ns, not significant