Fig. 1

Baseline expression levels of the TCR/CD3 complex, CD5 and c-CBL and phosphorylation levels of CD3ζ and ERK upon stimulation in relation to HLA-A2. a, b Expression levels of TCR/CD3 complex and CD28 in A2^pos and A2^neg primary CD8 T cells (a) or A2^pos and A2^neg J76 CD8αβ cells (b) engineered with TCRs of incremental affinities and analyzed under steady-state culture condition, in the absence of cognate antigen. c Quantification of CD3ζ and ERK1/2 phosphorylation in TCR-expressing A2^pos and A2^neg CD8αβ J76 cells after stimulation with NY-ESO-1 multimer, OKT3 (anti-CD3ε) antibody or PMA/Ionomycin. d Quantification of CD5 and c-CBL expression in A2^pos and A2^neg CD8αβ J76 cells. Direct comparison (bottom panel) of c-CBL expression in A2^neg versus A2^pos CD8αβ J76 cells for the indicated TCR variants by two-tailed, paired t test. a-d Data are means ± SD and representative of 5 to 15 independent experiments. TCR-transduced A2^pos cells versus A2^neg T cells are depicted as red versus blue symbols. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 and **** P ≤ 0.0001