Fig. 2

Kinetics of the expression of co-activating/inhibitory surface receptors upon affinity-increased TCR transduction in the absence of cognate peptide antigen. a, c Schematic representations of the phenotype characterization of A2^pos and A2^neg J76 CD8αβ cells (a) or A2^pos and A2^neg primary CD8 T cells from individual healthy donors (c) following affinity-increased TCR transduction, in the absence of antigen-specific stimulation. b and d Kinetic analyses of CD69, PD-1, TCR/CD3ε complex and c-CBL in redirected J76 CD8αβ cells (b) or of co-activating/co-inhibitory receptor expression in redirected primary CD8 T cells (d) from day 3 or 6 up to day 21 after TCR transduction. Data are representative of 2 to 4 independent experiments