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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Chronic TCR-MHC (self)-interactions limit the functional potential of TCR affinity-increased CD8 T lymphocytes

Fig. 5

Basal cytokine production and killing capacity in relation to HLA-A2. a Multiplex cytokine production data was performed on A2pos and A2neg primary CD8 T cells without (baseline) or with low dose of 0.1 nM A2/NY-ESO-1157-165 multimers (activated) during 24-h of culture. Cytokine concentrations are shown as heat map (left panel) or as relative concentrations to no-TCR variants after specific stimulation (right panel). b Schematic representation of the real-time quantitative killing assay by IncuCyte. A2pos (CRISPR/GFP) and A2neg (CRISPR/A2) primary CD8 T cells of affinity-increased TCRs were co-cultured with NA8 (A2pos/NY-ESO-1neg) target tumor cells (E:T ratio; 1:10), without or with low dose of 1 nM NY-ESO-1157-165 peptide (pp) during 4 days. c Representative images at 96 h (upper panels) and quantification (lower panels) of caspase 3/7-dependent apoptosis induced by tumor-redirected A2pos versus A2neg CD8 T cells are shown. Data are representative of 2 independent experiments

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