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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: Chronic TCR-MHC (self)-interactions limit the functional potential of TCR affinity-increased CD8 T lymphocytes

Fig. 7

Activation phenotype and basal killing capacity of tumor-redirected A2neg CD8 T cells in long-term co-cultures with NA8 tumor cells. a Schematic representation of the experimental design; A2neg (CRISPR/A2) primary CD8 T cells were co-cultured with either A2pos or A2neg NA8 tumor cells for 20 days in the absence of cognate antigen before being sorted and tested for their killing capacity. b Kinetics of the expression levels of co-activating/inhibitory receptors on A2neg CD8 T cells during long-term co-cocultures with A2pos or A2neg NA8 cells. c Representative images at 94 h (upper panel) and quantification (lower panel) of caspase 3/7-dependent apoptosis of NA8pos target cells induced by A2neg CD8 T cells previously co-cultured during 20 days with either A2pos or A2neg NA8 cells are depicted, using IncuCyte

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