Fig. 2

Involvement of vβ TCR CDR3 2, 3, 8.3 T cell families in the graft-versus-myeloma effect in myeloma bearing Balb/c mice treated by irradiation, Auto-BMT and then allogeneic lymphocyte infusion. Recipient mice were sacrificed when severe GvHD symptoms, myeloma symptoms or apathy were present. Flow cytometry staining was performed on cells from spleen and bone marrow at sacrifice. a Infiltration of MOPC MM cells in the bone marrow and spleen identified as CD138⁺CD4⁺ double positive cells. *p = 0.0006. **p = 0.0018 (Student t test). b Paraprotein serum IgA quantitation (μg/ml) by ELISA before Auto-BMT, 1 week after and at sacrifice. *p = 0.0003. **p = 0.005 (Student t test). c vβ(2 + 3 + 8.3)⁺ T cell populations in the graft-versus-myeloma effect. Shown are percentages of activated CD4⁺vβ(2 + 3 + 8.3)⁺ T cells (CD69+ within CD4⁺vβ(2 + 3 + 8.3)⁺ T cells) and activated CD8⁺vβ(2 + 3 + 8.3)⁺ T cells (CD69+ within CD8⁺vβ(2 + 3 + 8.3)⁺ T cells) in the spleen (left panel) BM (right panel) in the MM-Auto-BMT, MM-Auto-BMT + Allo naive vβ 2, 3, 8.3 (× 1) group, MM-Auto-BMT + Allo activated vβ 2, 3, 8.3 (× 1) or in healthy Balb/c mice. *p < 0.0001; **p < 0.0001; ***p < 0.05 (Student t test)